Abstract

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Groundwater, which serves as the major drinking water source to densely populated areas in Osogbo metropolis, are usually in close proximity to defective septic tanks. Faecal contamination of such water system is inevitable. This work investigated the faecal contaminants in eighty wells from five locations. Total coliform count (TCC) was determined using Most Probable Number (MPN) and membrane filtration; Escherichia coli was recovered using chromogenic media. Antibiotic susceptibility profile was obtained for E. coli isolates. All wells were > 15.24m from nearest septic tank; averaging 78m. Depth to water surface ranged between 9ft and 48ft with a mean of 23.8ft while 16.2% were deeper than 30ft to water surface. All samples (100%) had coliforms. TCC ranged between 1.0 x 102 and 4.0 x 105 cfu/ml (except Kasmo); with a mean 3.8 x 104 cfu/ml. Mean TCC for wells was highest in Igbona area and lowest for Oke-baale. Escherichia coli was isolated from 48.8% of samples. Five samples had two strains of E. coli as revealed on chromogenic media. The study recorded 100% resistance to ticarcillin and meropenem; 52.3% to tigecycline. Aztreonam and Colistin inhibited 92.6% and 91.0% of isolates respectively. Multidrug resistance was evident in 79.5% of isolates. The well water samples analyzed were neither safe for drinking nor put into such uses that may facilitate ingestion by humans. E. coli was most susceptible in-vitro to aztreonam and colistin, thus suggesting their use in the treatment of gastrointestinal syndromes resulting from consumption of contaminated groundwater.

Keywords: Indicator organism, public health, septic tank, well water contamination,